Syllabus
Course Summary:
Theoretical Training
Application of DNA chips in diagnosis of genetically determined diseases
Practical Training
Laboratory procedures in DNA chips preparation
Demonstration of instrumentation, basic stages of DNA chips preparation, samples and data management (BASE)
Laboratory procedures in experimental application of DNA chips
Experimental design, RNA isolation and analysis, RNA labelling, hybridisation
Data analysis
Chip scanning, data formats, data preprocessing and normalisation, statistical data analysis, functional annotation
Information: vstra@lf1.cuni.cz
Annotation
Practical Course of Aplication of DNA chip technology in diagnosis of genetic disorders
Annotation The course is meant for highly motivated students of 3. to 6. year of general medicine studies, who plan to continue as a PhD students, and for PhD students, who can take advantage of these techniques during their lab work. The maximum capacity is 5 students. Participants of the course will be selected from all applicants on the basis of a short letter of motivation (max. 200 words). Prerequisite of the credit assignment is the full-time presence during the course (approx. 10 hours). The course has been accepted by the Ph.D. studies Board in Biochemistry and Pathobiochemistry, preliminary acceptance was given by the Cell Biology and Pathology Board. Acceptance by other Boards depends on the individual agreement of the participant and the particular Board.
The exact terms of the courses:1) 18.11.2008, 2) 19.11.2008, 9-17 hour , at Laboratory for DNA chip technology, Department of Inborn Errors of metabolism (UDMP), building D, Ke Karlovu 2, Prague , information: vstra@lf1.cuni.cz, deadline for registration is 5.11.2008