Syllabus
Medical Microbiology - 3rd year - winter semester
1) Staphylococci - Identification of staphylococcal species using biochemical identification systems. Bound and free coagulase in differentiation of coagulase positive (e.g. S. aureus) and negative staphylococci.
2) Streptococci - Colony morphology of clinically significant streptococci and their differentiation among β-hemolytic and group of Viridans streptococci using PYR, CAMP and optochine tests.
3) Neisseria, Corynebacteria and Listeria - Colony morphology using enriched and selective media, C. diphtheriae - toxigenity test. Temperature dependent culture of L. monocytogenes.
4) Enterobacteriaceae - Laboratory diagnosis of significant species and strains using biochemical tests and reverse agglutination method.
5) Bordetella, Haemophilus and Pseudomonas - Fastidious nature of Haemophilus species and special enriched media. Specimen collection and diagnostic media to detect Bordetella species. Colony pigmentation and resistance of Pseudomonas aeruginosa strains.
6) Anaerobic bacteria - Anaerobic culture conditions. Overview of clinical material collected from patients with suspected anaerobic infection. Identification of clinically significant anaerobic agents using biochemical tests. Spore-forming anaerobes of the Clostridium genus.
7) Mycobacteria and Candida - Ziehl-Neelsen stain of acid-alcohol resistant mycobacteria; culture of medically significant mycobacterial strains. Yeasts and moulds - native prepration and culture of medically significant species.
8) Interpretation of laboratory results - Overview of laboratory methods applicable in diverse clinical situations. Interpretation of microbiological and serological results.
9) Parasitology - Microscopy and macroscopy in identification of clinically significant parasites. Summary of general parasitology.
10) Molecular methods in clinical microbiology - Overview of molecular methods applicable in medical microbiology laboratory - benefit and limitation.
11) Specimen collection and transport of clinical material - Applicable methods to collect and transport clinically significant clinical specimens. Gram staining procedure.
12) Processing of respiratory and urogenital specimens to detect infectious agents.
13) Processing of gastrointestinal specimens - Diagnostics of bacterial (Salmonella, Shigella, Campylobacter) and other agents. Transmission of the agent and relevant therapeutical properties. Review - overview of relevant clinical material and diagnostic procedures.
14) Consultation, credit - Completed credit test and absolute presence is required to receive the credit.
Annotation
Basic principles of general microbiology, patogenetic mechanisms, special bacteriology, mycology, virology and parasitology, principles of antimicrobial therapy, antiinfectious immunity and specific and nonspecific immunomodulation, clinical microbiology with selection of laboratory techniques, interpretation, differential diagnostics.