Syllabus
Syllabus - Stem Cells and Biomaterials in Regenerative Medicine
Short anotation:
Embryonic and tissue-specific stem cells, biomaterials for tissue engineering (scaffolds, nanofibers, spinal cord repair), GMP facilities, clinical studies
Day 1 Place Department of Neuroscience 2. LF UK, Motol hospital, 2D
Lectures Introduction to stem cell biology and biomaterials. Jendelová
Safety, Laboratory work. Růžička
Practical course Basic cell culture laboratory - laminar flow cabinet, centrifuge, incubator, laboratory shaker etc.
Work in a biohazard box - sterility, single use and sterilizable materials, waste management.
Basic cell culture techniques. Růžička
Day 2 Place Department of Neuroscience 2. LF UK, Motol hospital, 2D
Lectures Embryonic stem cells and induced pluripotent cells. Romanyuk
Practical course MSC isolation from rat bone marrow (femur, tibia), preparation of primary cultures (video with comment, 15 min)
Primary culture parameters, importance of reagents and supplements (medium, FBS, PBS, trypsin), mesenchymal stem cell (MSC) characteristics (25 min)
Practical session
Work in a flow box, preparation of materials for tissue engineering (nanofibers, hydrogels, hydroxyapatite), seeding of MSCs
Cell culture (media exchange, passaging, cell counting, basic microscopy)
Media (15 min)
Checking of confluence (10 min)
Passaging, cell counting (20 min)
Seeding stem cells on materials (10 min)
Růžička
Day 3 Place Department of Neuroscience 2. LF UK, Motol hospital, 2D
Lectures Neural stem cells, their isolation, characterization and use in experiments. Jendelová
Hematopoietic stem cells, their isolation, purification and use in clinical medicine. Konrádová-Langkramer
Practical course seminar
Biomaterials for implantation into different models of spinal cord injury (SCI)
Implantation of hydrogels into a hemisection model of SCI (video with comment, 30 min)
Balloon compression lesion model of SCI (video with comment, 15 min)
Behavioural tests to evaluate motor and sensory function in a rat model of SCI (video with comment, 20 min)
Růžička
Day 4 Place Department of Neuroscience 2. LF UK, Motol hospital, 2D
Lectures Mesenchymal stem cells and their use in regenerative medicine. Jendelová
Cell labeling with iron-oxide nanoparticles and their monitoring by MR imaging. Jendelová
Practical course Practical session
Microscopy - Imaging of cultures and seeded materials (Tuesday work results 20 min). Basic histological staining (DAPI, Phaloidin, GFP) (15 min)
Image analysis software - Image J (cell counting, area summation), Axiovision Wizard software
Image analysis - hydrogel properties, cell attachment (10 min)
Nanofibers - idea of orientation Hydrogels - surface characteristics, porosity, modifications (10 min)
Confocal vs. fluorescence microscopy (15 min)
Růžička
Day 5 Place Innovation Biomedical Center
Institute of Experimental Medicine
Videnska 1083, Prague 4
Lectures Clinical trials with mesenchymal stem cells. Lesný
Advanced therapeuticals and cell manipulation under Good Manufacturing Practice conditions.
Practical course Clean rooms. Lesný
Annotation
Embryonální, fetální, mezenchymální a tkáňově specifické kmenové buňky; teoretické základy, vývoj v oboru.
Praktické aplikace kmenových buněk v terapii závažných onemocnění.