Syllabus
Lecturers
Lecturer: assoc. prof. RNDr. Věra Králová, Ph.D.
Organisation of living systems – definition of life, cell theory; prokaryotic and eukaryotic cellsModels in biomedicineDNA replicationTranscription,post-transcriptional modifications of RNATranslation, post-translational modifications of proteins
Organelles of eukaryotic cellsCell membranes, membrane transport
Mitochondria – structure and function, ATP productionCytoskeleton – microtubules, microfilaments, intermediate filaments, cellular motility
Regulation of cellular functions – principles of cell signaling, signals, receptors, signaling pathways, regulation of gene expressionCell cycleCell death
Genes, genomes, variability of individuals. Mutations, polymorphisms, reparatory mechanisms.Organisation of human genome, chromosomes, chromatin structureMeiosis
Practical courses & seminars
Lecturer: Prof. PharmDr. Emil Rudolf, Ph.D. 1. Microscopic techniques I.1.1 General terms and conditions of the biology course1.2. Imaging modalities in biological applications1.3 Light microscopy in biological applications 2. Advanced microscopic techniques II.2.1 Contrast enhancing microscopy (phase contrast, DIC, fluorescence)2.2 Native mount of human cells 3. Cellular membranes – fusion and osmosis3.1 Selected functions of biological membranes 3.2 Osmosis in plant cells – native mount 4. Cell motility4.1 E-learning – cell motility4.2 Motility in eukarytotic cells – cyclosis, ciliated and flagellar movement4.3 First progress test 5. Cell cultures in biomedicine5.1 Modeling in biomedicine (3R principle, in vitro models)5.2 Cultivation of cells in vitro5.3 Introduction to the cell culture laboratory 6. Information sources and their relevance in biomedicine6.1 Scientific information in biomedicine – the structure of scientific article6.2 Electronic bibliographic database (SCOPUS,PUBMED, WOS) 7. Cellular stress7.1 Mechanisms, factors and outcomes of cellular stress7.2. Cultured cells in suspension, vital staining 8. Cell cycle and cell division8.1 Techniques of cell cycle and proliferation measurement8.2 Mitotic figures in plant and animal cells – permanent mount8.3 Mitotic and proliferation indexes in cultured cells8.4 Second progress test 9. Advances in biosciences (seminar)9.1 Student presentations (biodentistry oriented) 10. Summary of the course10.1 CREDIT
Annotation
Definition of life, organisation of living systems. Molecules in cells, structure and function of proteins and nucleic acids.
Cell theory, prokaryotic and eukaryotic cells. The cell nucleus, organisation of DNA, chromosomes.
DNA replication and repair. Transcription and translation of genetic information, RNA processing, posttranslational modification.
The cellular membranes, structure and function. Transport across biomembranes, cell membrane fusion.
The endoplasmic reticulum, the Golgi apparatus. The mitochondrion, production of ATP.
The cytoskeleton. Cell growth and cell division.
Regulation of cellular functions. Cell death, necrosis, apoptosis.