Laboratorní metody. Přehled diagnostických metod používaných pro rutinní praxi a jejich principy, odběr klinických vzorků, transport, preanalytická fáze zpracování klinických materiálů, správná laboratorní praxe (např. externí hodnocení kvality, akreditace laboratoří, doporučení), citlivost, specificita, PPV, NPV, RLU křivka, analytická citlivost, klinická citlivost diagnostických testů, in vitro diagnostika a spolehlivost komerčních setů.
\r\nPatogeneze virových onemocnění
HBV, HAV, HCV, a další viry vyvolávající hepatitidy
Herpetické viry - HSV1,2, CMV, EBV, HHV6, HHV8, VZV
Chřipka a další respirační viry
HIV, HPV
Virové gastroenteritidy - rotaviry, noroviry, astroviry
Rubeolla, příušnice, spalničky
Zoonózy - virus vztekliny, virus chřipky savců a ptáků, paramyxoviry, anelloviry
Hospodářsky významné virové infekce zvířat - pestiviry, virus slintavky a kulhavky, virus Newcastelské choroby ptáků, herpesviry zvířat
Retroviry zvířat, circoviry, parvoviry, coronaviry
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Basic principles of epidemiology and molecular epidemiology of viruses. Types of epidemiological studies, terms prevalence, incidence, mortality, morbidity, principals of utilization of application of molecular epidemiology in virology. How to prove aetiology of viral infection of a disease, eradication of diseases, epidemics, pandemics. Laboratory methods, focused on those used in the routine practice, sampling of patients, transport of clinical samples, pre analytic and post analytic processing of clinical samples, good laboratory practice, certification of laboratories, external control of quality. Characteristics of tests for routine viral detection.
\r\nPathogenesis of viral infections
HBV, HAV, HCV, and other viruses causing hepatitis
Herpetic viruses - HSV1,2, CMV, EBV, HHV6, HHV8, VZV
Influenza and other respiratory viruses
HIV, papillomaviruses
Viral gastroenteritis - rotavirus, norovirus, astrovirus
MMR
Zoonosis - rhabdovirus, influenza
Viruses of cats and dogs
Animal retroviruses, parvoviruses, circoviruses, coronaviruses
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Basic principles of epidemiology and molecular epidemiology of viruses. Types of epidemiological studies, terms prevalence, incidence, mortality, morbidity, principals of utilization of application of molecular epidemiology in virology. How to prove aetiology of viral infection of a disease, eradication of diseases, epidemics, pandemics. Laboratory methods, focused on those used in the routine practice, sampling of patients, transport of clinical samples, pre analytic and post analytic processing of clinical samples, good laboratory practice, certification of laboratories, external control of quality. Characteristics of tests for routine viral detection.
Pathogenesis of viral infections HBV, HAV, HCV, and other viruses causing hepatitis Herpetic viruses - HSV1,2, CMV, EBV, HHV6, HHV8, VZV Influenza and other respiratory viruses HIV, papillomaviruses
Viral gastroenteritis - rotavirus, norovirus, astrovirus MMR
Zoonosis - rhabdovirus, influenza Viruses of cats and dogs Animal retroviruses, parvoviruses, circoviruses, coronaviruses
The lecture course will focus primarily on viruses, which cause diseases in humans and animals and which are diagnosed as a part of routine medical practice. Our main emphasis will be on diagnostic principles and epidemiological aspects.
At the beginning of the course, we will outline basic definitions used in epidemiology and molecular epidemiology. We will characterize regulations for evaluating diagnostic methods and commercial tests, describe methods and rules of work in a virological laboratory as well as define principles of good laboratory practice.
In subsequent lectures we will focus on groups of viruses that cause diseases with similar clinical manifestation (e.g. respiratory viruses or viruses causing hepatitis) and specify their mode of transmission, incubation period, type of infection, clinical symptoms, diagnostics, prevention and prophylaxis.
Lectures concerning viruses with veterinary importance will be focused on zoonotic viruses or viruses with zoonotic potential, viruses with high economical importance and viruses with interesting or unknown pathogenesis of the disease.