Syllabus
Introduction (why protein engineering?, antibodies as an example of engineered proteins), DNA sources and manipulation Expression plasmids, cloning/mutagenesis techniques (restriction sites, Gateway, Gibson assembly, In fusion)
3. Heterologous expression systems (prokaryotic, yeast, insect, mammalian) - transfection, cultivation, disruption
4. Basics of amino acid/protein structure, protein purification techniques I (affinity tags, ion exchange, hydrophobic, size-exclusion)
5. Protein purification techniques II, protein analysis (purity, concentration, dispersity, function, structure)
6. Repetition/back-up
7. Cell-free synthesis
8. Creating Diversity / Libraries epPCR, degenerate oligo design, gene shuffling, library preparation
9. Expanded / Alternative Genetic Code codon reassignment
10. Screen vs Selection towards new function display techniques (Phage, ribosomal, mRNA/cDNA), compartments
11. Minimal cells genome reduction
12. Synth Bio metabolic engineering; gene circuits