Lectures

Aims and specific features of bioanalysis of drugs
Withdrawal and storage of samples of body fluids before analysis
Immunochemical methods of drug monitoring (enzymoimmunoanalysis, radioimmunoanalysis)
Physico-chemical methods (chromatographic, spectral, electroanalytical)
Techniques and procedures of adjustments of samples of body fluids before analysis (Liquid-liquid extraction, SPE, protein precipitation, etc.)
Chromatographic methods with a stress on HPLC
Problems of interference of the biological matrix
Interpretation of results of the found drug levels in body fluids
Therapeutic Drug Monitoring
Analytical toxicology
The use of mass spectrometry in the field of Bioanalysis

Seminars and Practical trainings

Adjustments of samples of blood, plasma, urine before analysis with the use of physico-chemical methods
Liquid-liquid extraction, SPE, protein precipitation, optimization of procedures of isolation of selected drugs from samples of body fluids
Use of TLC as a screening tool in drug poisonings
Use of HPLC in bioanalysis, optimization of chromatographic conditions, selection of the detector, selection of the internal standard, interference of the residues from the biological matrix, verification of the correctness and precision of the elaborated methodology
Bioanalysis of drugs utilizing enzymoimmunoanalysis and radioimmunoanalysis
Therapeutic drug monitoring
Analytical toxicology
The use of mass spectrometry in the field of Bioanalysis of drugs