Lecturers

Lecturer: assoc. prof. RNDr. Věra Králová, Ph.D.

Organisation of living systems – definition of life, cell theory; prokaryotic and eukaryotic cells
Models in biomedicine
DNA replication
Transcription,post-transcriptional modifications of RNA
Translation, post-translational modifications of proteins
Organelles of eukaryotic cells
Cell membranes, membrane transport
Mitochondria – structure and function, ATP production
Cytoskeleton – microtubules, microfilaments, intermediate filaments, cellular motility
Regulation of cellular functions – principles of cell signaling, signals, receptors, signaling pathways, regulation of gene expression
Cell cycle
Cell death
Genes, genomes, variability of individuals. Mutations, polymorphisms, reparatory mechanisms.
Organisation of human genome, chromosomes, chromatin structure
Meiosis

Practical courses & seminars

Lecturer: Prof. PharmDr. Emil Rudolf, Ph.D.

1.            Microscopic techniques I.
1.1          General terms and conditions of the biology course
1.2.         Imaging modalities in biological applications
1.3          Light microscopy in biological applications

2.            Advanced microscopic techniques II.
2.1          Contrast enhancing microscopy (phase contrast, DIC, fluorescence)
2.2          Native mount of human cells

3.            Cellular membranes – fusion and osmosis
3.1          Selected functions of biological membranes
3.2          Osmosis in plant cells – native mount

4.            Cell motility
4.1          E-learning – cell motility
4.2          Motility in eukarytotic cells – cyclosis, ciliated and flagellar movement
4.3          First progress test

5.            Cell cultures in biomedicine
5.1          Modeling in biomedicine (3R principle, in vitro models)
5.2          Cultivation of cells in vitro
5.3          Introduction to the cell culture laboratory

6.            Information sources and their relevance in biomedicine
6.1          Scientific information in biomedicine – the structure of scientific article
6.2          Electronic bibliographic database (SCOPUS,PUBMED, WOS)

7.            Cellular stress
7.1          Mechanisms, factors and outcomes of cellular stress
7.2.        Cultured cells in suspension, vital staining

8.            Cell cycle and cell division
8.1          Techniques of cell cycle and proliferation measurement
8.2          Mitotic figures in plant and animal cells – permanent mount
8.3          Mitotic and proliferation indexes in cultured cells
8.4          Second progress test

9. Advances in biosciences (seminar)
9.1 Student presentations (biodentistry oriented)

10. Summary of the course
10.1 CREDIT