During the first part of the course, students will first learn basic laboratory tasks such as preparation and sterilization of solutions, buffers and microbiological media. It also introduces the basics of sterile work, inoculation and cultivation of microorganisms on solid and liquid media and monitoring of the growth curve.

In the second part of the course, students will learn the methods needed to work with DNA, such as plasmid DNA electroporation into bacteria, its growth and preparation of bacteria, and electrophoretic verification of isolated plasmid DNA including restriction analysis. Plasmid DNA thus obtained will be used as a template for in-vitro cloning of the transformation cartridge using PCR with primers according to its own design. The transformation cartridges will then be transformed into a yeast strain and the success of the prepared gene fusion will be verified again using PCR with primers according to their own design.

The third part of the course is devoted to analyzing prepared strains. Genetically modified yeast strains will be analyzed using a fluorescent microscope, spectrofluorimeter and flow cytometry. For these analyses will be used instrumentation in BIOCEVu in Vestci near Prague.