Introduction (why protein engineering?, antibodies as an example of engineered proteins), DNA sources and manipulation
Expression plasmids, cloning/mutagenesis techniques (restriction sites, Gateway, Gibson assembly, In fusion)

3. Heterologous expression systems (prokaryotic, yeast, insect, mammalian) - transfection, cultivation, disruption

4. Basics of amino acid/protein structure, protein purification techniques I (affinity tags, ion exchange, hydrophobic, size-exclusion)

5. Protein purification techniques II, protein analysis (purity, concentration, dispersity, function, structure)

6. Repetition/back-up

7. Cell-free synthesis

8. Creating Diversity / Libraries

epPCR, degenerate oligo design, gene shuffling, library preparation

9. Expanded / Alternative Genetic Code

codon reassignment

10. Screen vs Selection towards new function

display techniques (Phage, ribosomal, mRNA/cDNA), compartments

11. Minimal cells

genome reduction

12. Synth Bio

metabolic engineering; gene circuits