Lectures

Professor: prof. PharmDr. Emil Rudolf, Ph.D.

Introduction, modelling in biomedicine
Properties of eukaryotic cells in multicellular organisms
Nucleus- structure and function.
DNA synthesis and expression (transcription and translation)
Biological membranes - structure and function, medical relevance
Cytoskeleton - structure and function, medical relevance
Cell cycle and reproduction, regulation, signalling
Cell stress – pathways and responses
Cell death – types, principles, regulation

Practical courses

Instructors:

Prof. Dr. Emil Rudolf, Ph.D.
Assoc prof. Dr. Věra Králová, Ph.D.
Dr. Kateřina Dvořáková

Introduction to the course, information sources, microscopy
Rules and requirements of the course
Scientific information in biomedicine – biology (types, databases)
Introduction to light microscopy
Mock test – 1st lecture
Microscopy of living cells – split class (group A)
Group A - Progress test 1
Contrast enhancing microscopies in observation of living cells
Group B - Fluorescence – e-learning course and Progress test 2 (Moodle)
Microscopy of living cells – split class (group B)
Group B - Progress test 1
Contrast enhancing microscopies in observation of living cells
Group A - Fluorescence – e-learning course and Progress test 2 (Moodle)
Cell cultures in biomedicine
Progress test 3
Cultivation of cells in vitro
Introduction to the tissue culture laboratory
Cellular membranes – fusion and osmosis
Progress test 4
Osmosis in plant and animal cells
Cell fusion
Cell motility – split class (group A)
Group A - Progress test 5
Cytoplasmic streaming, Flagellar and ciliated motility
Group B - Cell motility – e-learning course and Progress test 6 (Moodle)
Cell motility – split class (group B)
Group B - Progress test 5
Cytoplasmic streaming, Flagellar and ciliated motility
Group A - Cell motility – e-learning course and Progress test 6 (Moodle)
Cell proliferation and its measurement
Progress test 7
Techniques of cell cycle and proliferation measurement
Mitotic and proliferation indexes in cultured cells
Cellular stress – split class (group A)
Group A - Progress test 8
Cultured cells in suspension, vital staining
Cell stress and demise in human cells
Group B - Tests of cytotoxicity in vitro – e-learning course and Progress test 9 (Moodle)
Cellular stress – split class (group B)
Group B - Progress test 8
Cultured cells in suspension, vital staining
Cell stress and demise in human cells
Group A - Tests of cytotoxicity in vitro – e-learning course and Progress test 9 (Moodle)
Meiosis and genetic recombination
Progress test 10
Meiosis and gene linkage analysis- interactive workshop
Meiosis in cells of locust