Staining methods, manipulation with laboratory mouse

- blood smear, thick drop

- staining of dry smears by Giemsy-Romanowski method

- fast staining by Dade Diff-Quik

- manipulation with laboratory mouse, infection with Trypanosoma brucei, Plasmodium berghei

Fluorescent staining methods

- vital staining with acridine orange

- nuclear staining by Hoechst or DAPI

- pH sensitive fluorescent probes

- FITC labeling

- immunofluorescence on wet mounts

Basic cultivation techniques

- preparation of antibiotic stock solutions

- isolation of the Tritrichomonas foetus axenic culture using a migration tube

- passage of axenic cultures, sterility test

- freezing techniques, cryopreservation of protozoa

Cloning

- Isolation of clones by dilution method (Tetrahymena pyriformis)

- isolation of clones by limiting dilution method (Trichomonas vaginalis)

- isolation of clones on agar plates (Crithidia fasciculata)

Quantitative work with protists

- Work with Burker's counting chamber

- calculation of cell concentration

- evaluation of the growth experiment, calculation of doubling time from growth curves

ELISA

- use in diagnostics

- test for the presence of antibodies against Toxoplasma gondii

PCR

- use in diagnostics

- PCR test for human pathogens / commensals

- DNA isolation methods

- DNA electrophoresis, visualization

Preparation and isolation of recombinant protein

- work with bacterial culture, conditions of induction and expression of foreign protein

- protein isolation in native and denatured form by affinity chromatography

- analysis of protein sample by electrophoresis

- electrophoretic methods

In 2020, new methods will be introduced from the insect vector and helminthology fields.