1) Construction of Recombinant Microorganisms and Molecular Evolution (methods of recombinant DNA; PCR; cloning of structural genes; mutagenesis in vivo; mutagenesis in vitro; gene shuffling; molecular evolution and its importance; ...) - Dr. Štěpánek

2) Microbial Expression Systems (homologous and heterologous expression systems; modification of heterologous product; metabolic burden; bacterial expression systems; yeast expression systems; non-conventional microbial expression systems; antimicrobial peptide production; ...) - Dr. Štěpánek

3) Microbial Metagenomics and Metagenomic Libraries ("OMICS" methods; metagenome; isolation of environmental DNA; construction and screening of environmental metagenomic libraries; analysis of primary metagenomic bioinformation; directed metagenomics; synthetic metagenomics; "global" metagenomic projects; ...) - Dr. Štěpánek

4) Application of Metagenomics in Biotechnology (metagenomics of extreme environments & biotechnology; metagenomic hydrolases, oxidoreductases and lyases; metagenomics of "small bioactive molecules"; ...) - Dr. Štěpánek

5) Synthetic Biology (metabolic engineering and bioprocess design; metabolic factories - Escherichia coli, Corynebacterium glutamicum; perspectives of synthetic biology; design of synthetic structural genes; regulation of gene expression using synthetic biology; synthetic biology softwares; synthetic genes & industry; production of "biochemicals"; glycoengineering; chemical synthetic biology; synthetic ecosystems; ...) - Dr. Štěpánek

6) Biosensors (biological components and transduction elements of biosensors; aptasensors; bacteriophage biosensors; EMWCB technology; enzyme biosensors; biodetection of heavy metals, inhibitors, toxins or pollutants; biosensing of microbial pathogens; geochemical biosensors; ...) - Dr. Štěpánek

7) Application of Recombinant Microorganisms in Bioremediation (bioremediation technology; principles and mechanisms of bacterial bioremediation; pollutants-degrading microorganisms; degradation of APIs; ...) - Dr. Palyzová

8) Biotechnology Application of Microbial Pathogenicity Factors (pathogenicity and virulence; microbial biofilms; quorum sensing; quorum quenching; mechanisms of bacterial metal-siderophore transport systems; siderophore-drug conjugates; ...) - Dr. Palyzová

9) Heterologous Protein Production by Yeast Expression Systems (eukaryotic expression systems; yeast expression vectors; construction and screening strategy of yeast expression systems; overexpression of recombinant genes in yeasts; intracellular and extracellular production of recombinant proteins; ...) - Dr. Marešová

10) Cultivation of Microorganisms and Fermentation Equipment (culture parameters; biofilm - microbial life on surfaces; submerged fermentation; batch culture; fed-batch culture; continuous chemostat culture; bioreactors; ...) - Dr. Palyzová