Please note, the lectures are given in Czech language only.

The lecture will be recorded by the teacher (sound + projected image). These materials will be accessible online.

TOPICS:

Structure and properties of DNA, RNA and their subunits

Conformational polymorphism, sequence polymorphism, structural anomalies and DNA topology
Electron microscopy of nucleic acids
Denaturation and reassociation analysis of nucleic acids, reassociation probes and matrices (microarrays)

Structure and properties of proteins and their subunits

Determination of molar mass of protein molecules
Protein mass spectrometry

Overview of separation and purification techniques

Sedimentation analysis of biopolymers, moving interface method, differential, isokinetic and isopycnic centrifugation
Electrophoretic analysis and separation of biopolymers including pulse electrophoresis
Chromatography

Determination of concentration of information biopolymers (spectrophotometry, fluorimetry and colorimetry)

DNA, RNA and protein sequencing

Details of quantitative polymerase chain reaction (Real-time PCR or qPCR)

Determination of structure and interactions of DNA, RNA and proteins (basics of MS, NMR, X-ray diffraction)

Methods of monitoring gene expression, resp. representation of various RNAs or proteins in biological objects

Much of the practical exercises use PyMOL. It is advisable to carry your own notebook with you (the teacher announces in advance when it is needed).