Microbiology, DENTISTRY - Syllabus

Lectures, Clinical microbiology:

1. Introduction, history of clinical microbiology, evolution of microbes, bacterial genetics (viruses, bacteria, parasites, mechanisms of horizontal gene transfer - transformation, transduction, conjugation, specific genetic elements - insertion sequences, transposons, integrons, etc.)

2. Principles of general microbiology, factors responsible for virulence, pathogenesis of infectious diseases (structure of virus, virus living cycle, basic knowledge of immunity response, barriers, bacterial toxins, adhesins, mechanisms of escape against immunity, etc.)

3. Review of clinically important G+ bacteria with special focus on oral cavity (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Streptococcus spp., Enterococcus spp., Staphylococcus spp., Bacillus spp., Corynebacterium spp., etc.)

4. Review of clinically important G- bacteria with special focus on oral cavity (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Neisseria spp., Haemophilus spp., Enterobacteriaceae, Vibrio spp., Campylobacter spp., Helicobacter spp., Pseudomonas and other non-fermenting rods, Legionella spp.)

5. Review of clinically important bacteria (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Mycobacterium spp., Nocardia spp., Actinomyces spp., Chlamydia spp., Mycoplasma spp., Rickettsia spp., anaerobic bacteria - review of the most important species, e.g. Clostridium difficile, C. perfringens, C. tetani, C. botulinum, Peptostreptococcus spp., Fusobacterium spp.)

6. Review of clinically important parasites 1 (physiology of parasite, living cycle, vectors, diseases, diagnostics, treatment; protozoology, Trypanosoma spp., Leishmania spp., Trichomonas vaginalis, Giardia intestinalis, Entamobea histolytica, Plasmodium spp., Toxoplasma gondii, Balantidium coli, Cryptosporidium parvum)

7. Review of clinically important parasites 2 (physiology of parasite, living cycle, vectors, diseases, diagnostics, treatment; helminthology, medical entomology, Fascilopsis buski, Paragonimus westermani, Clonorchis sinensis, Schistosoma, spp., Taenia spp., Echinococcus spp., Hymenolepis nana, Nematodes)

8. Review of clinically important fungi with special focus on oral cavity (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, therapy; Candida spp., Craptococcus spp., Aspergillus spp., Penicillium spp., dermatomycoses, Hystoplasma spp., Pneumocystis jirovecii)

9. Antibiotics (basic groups of antibiotics, mechanism of action, molecules; beta-lactams, glycopeptides, polymyxins, amynoglykosides, quinolones, sulfonamides, chloramphenicol, etc.)

10. Mechanisms and epidemiology of antibiotic resistance (mechanisms of bacterial resistence, clonal spread of resistant bacteria, horizontal transfer of genes - mobilisation of genes, mobile genetic elements, integrons, co-resistnace, cross-resistance)

11. Hospital-acquired infections (definition, examples of the most important pathogens, resistence bacteria as a causative agents of hospital-acquired infections - MRSA, VRE, resistant Gram-negative rods - production of extended-spectrum beta-lactamases, carbapenemases)

12. Review of viral diseases 1 (structure of virus, pathogenesis, epidemiology, diagnostics, prevention, therapy; papillomaviruses, polyomaviruses, adenovirus, herpesviruses, poxviruses, picornaviruses, coronaviruses, paramyxoviruses, influenza virus, rhabdoviruses, filoviruses, reoviruses, togaviruses and flaviviruses, togaviruses, bunyaviridae)

13. Review of viral diseases 2 (structure of virus, pathogenesis, epidemiology, diagnostics, prevention, therapy; retroviruses - HIV, hepatitis viruses)

14. Oral microbiology

Practical courses, winter semester 2022/2023

6.10. Introduction, Safety, General Microbiology, Diagnostic Process

13.10. Pre-analytical Phase, Specimen Collection, Disinfection and Sterilization

20.10. Microscopy

27.10. Cultivation I.

3.11. Cultivation II. - Identification, ATB Susceptibility, Anaerobes

10.11. Immunology Methods, Molecular Genetic Methods

(17.11. public holiday)

24.11. Gastro-Intestinal Tract Infections, Urinary Tract Infections

1.12. Respiratory Tract Infections

8.12. Infections of Skin, Bones and Joints, Sexually Transmitted Infections

15.12. CNS infections, Bloodstream Infections

22.12. Review of Clinically Important Parasites

Review of Clinically Important Fungi

5.1. Credit