Natural organic dyes are used primarily for the production of organic pigments, paints and textile dyeing or as lakes (insoluble form). These dyes are extracted from plants and animals and contain various organic compounds which are typical for locality and climatic conditions of their origin.
These substances are structurally very similar and thus for their identification, analytical separation and spectroscopic techniques are used. Methods such as Raman or IR spectroscopy are outstanding in their non- destructivity and accuracy, but vibration bands of individual dyes could be overlapped by vibration band matrixes.
Separation techniques such as thin layer chromatography, capillary zone electrophoresis, and micellar electrokinetic chromatography are considered to be destructive methods, but they have a power to resolve the dyes from their matrix with high separation efficiency. This could be used for precise identification of dyes.