Broadband heteronuclear dipolar recoupling without H-1 decoupling in solid-state NMR using simple cross-polarization methods
2010
Abstract
Heteronuclear dipolar recoupling experiments without H-1 decoupling based on simple cross polarization are introduced for applications in biological solid-state NMR. It is shown that standard or adiabatic variants of the cross-polarization experiment with irradiation on the low-gamma (e. g., C-13, N-15) spins even at modest spinning frequencies enable efficient band-selective or broadband dipolar recoupling without the need for intense H-1 decoupling.
This facilitates experiments on expensive isotope-labelled protein samples for which sample heating by intense H-1 decoupling may lead to sample detoriation.