Some representatives of the bivalve family Sphaeriidae are assumed to be polyploid. In this study, 11 sphaeriid species inhabiting central Europe were studied karyologically, 10 of them for the first time.
Analysis revealed high chromosome numbers (from 140 to 240). To elucidate the origin of high chromosome numbers, DNA contents were measured by flow cytometry in 5 of the studied species and, for comparison, in S. corneum and S. nucleus, which are known to be diploid (2n=30).
Species with high chromosome counts yielded very similar DNA contents that are not higher than in the related species with low diploid numbers. This finding contradicts a possible origin of these species by recent polyploidization or hybridization of related species.