Aluminium ions inhibit the formation of diacylglycerol generated by phosphatidylcholine-hydrolysing phospholipase C in tobacco cells
2010
Abstract
Aluminium ions have been recognized as a major toxic factor for crop production in acidic soils. This study aimed to assess the impact of Al on the activity of phosphatidylcholine-hydrolysing phospholipase C (PC-PLC), a new member of the plant phospholipase family.
We observed a significant decrease of labelled diacylglycerol (DAG) in cells treated with AlCl3. The growth of pollen tubes was retarded in the presence of Al and this effect was accompanied by the decrease of labelled DAG similar to the case of the BY-2 cell line.
The growth of pollen tubes arrested by Al was rescued by externally added DAG. Our observation strongly supports the role of DAG generated by PC-PLC in the response of tobacco cells to Al.