Abstract
We studied the influence of dyslipidaemic diets on liver impairment and bone metabolism and bone rigidity. Male Wistar rats were divided into the following 4 groups.
Diets used were: standard laboratory diet, diet enriched with 4% of cholesterol, diet without choline and methionine and standard diet enriched with 1% of orotic acid. The rats were sacrificed by exsanguination after 28 days.
Then post mortem bone mineral density was measured using dual energy X-ray absorptiometry. The femurs were extracted from animals and broken in a controlled manner using biomechanical testing.
Bone homogenate was analyzed for osteocalcin, procollagen type I N-terminal propeptide. The findings suggest that diet with orotic acid decreased bone collagen formation, did not influence bone mineral density but increased bone rigidity.
The other diet without choline and methionine leads to higher bone mineral density and procollagen type I N-terminal propeptide, but without improvement of fracture resistance.