Novel Stably Transfected Gene Reporter Human Hepatoma Cell Line for Assessment of Aryl Hydrocarbon Receptor Transcriptional Activity: Construction and Characterization

Abstrakt

We constructed stably transfected gene reporter cell line AZ-AHR, allowing measurement of aryl hydrocarbon receptor (AhR) transcriptional activity. Human hepatoma HepG2 cells were transfected with a construct containing several AhR binding sites upstream of luciferase reporter gene.

We prepared 12 clones and we characterized the best five in responsiveness to TCDD.

Klíčová slova

• Ah receptor
• calux-bioassay
• induction
• CYP1A1
• expression
• 3-methylcholanthrene
• plasmids
• agonist
• system