Two-column Sequential Injection Chromatography - New approach for fast and effective analysis and its comparison with gradient elution chromatography
Abstract
This work presents novel approach in low-pressure chromatography flow systems - two-column Sequential Injection Chromatography (2-C SIC) and its comparison with gradient elution chromatography on the same instrument. The system was equipped with two different chromatographic columns (connected to selection valve in parallel design) for isocratic separation and determination of all components in composed anti-inflammatory pharmaceutical preparation (tablets).
The sample was first injected on the first column of length 30mm where less retained analytes were separated and then the sample was injected on the second column of length 10mmwhere more retained analytes were separated. The new two-column chromatographic set-up shows evident advantages as compared with single-column gradient method.