Effect of phytic acid and inositol on the proliferation and apoptosis of cells derived from colorectal carcinoma
Abstract
In our project we try to characterize the effect of phytic acid (IP6) as a potential adjuvant in treatment of colorectal carcinoma. There is some evidence that myo-inositol (Ins) can potentiate anticancer effects of IP6.
Therefore we tested both IP6 and Ins individually and in combination on human cell lines HT-29, SW-480 and SW-620 derived from the colorectal carcinoma in different stages of malignancy. The effect of tested chemicals on the cells was measured using WST, BrdU, Brilliant Blue and caspase-3 activity.
We tested IP6 and Ins at three concentrations: 0.2, 1 and 5 mM during 24, 48 and 72 hours. We found that all employed concentrations of IP6 or IP6/Ins decreased proliferation of all cell lines.
Metabolic activity and protein content of treated cells differed in response to IP6 and IP6/Ins treatment. We found the maximum increase in caspase-3 activity in HT-29 (5mM) and in SW480 (0,2 mM) with clear indication of Ins enhancing the proapoptotic effect of IP6 in all cell lines.