Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements

Abstract

For the first time, simultaneous monitoring of fluorescence and absorbance using Bead Injection in a Lab-on-valve format was used. Cells immobilized on a stationary phase, are exposed to reagents under well-controlled reaction conditions.

The applicability of this technique is demonstrated by the development of a Bead Injection method for automated measurement of cell density and intracellular hydrogen peroxide.

Keywords

• lab-on-valve
• bead injection
• flourescence
• absorbance
• intracellular H2O2 measurements