Abstrakt
The aim of our study was to investigate the effect of amlodipine on bone metabolism in male rats. Amlodipine (0.3 mg/100 g BW; gavage) was administered to 8 rats for 8 weeks.
Control group (n=8) received aqua pro inj.. Bone marker concentrations of CTX-I and PINP in serum, and BALP in both serum and bone homogenate were measured by ELISA.
We investigated expression of BMP-2 in tibia using Western blot, and bone mineral density was measured by DXA in lumbar and caudal vertebrae and in femoral areas. Femurs were used for biomechanical testing.
After 8 weeks of amlodipine administration there was decrease in serum levels of BALP (p=0.0009) and CTX-I (p=0.003), and level of BALP in bone homogenate (p=0.026) compared to controls. Expression of BMP-2 was increased after amlodipine administration.
Our findings suggest that amlodipine has retarding influence on bone metabolism in rats by decreasing bone turnover, which probably in consequence increases expression of BMP-2.