This study presents the results of the analysis of mtDNA bone samples morphologically determined to be aurochs (Bos primigenius) from four various archaeological finds in the Czech Republic (Central Europe). The results from two of them - the Pleistocene sample from Praha- Řeporyje and the Neolithic sample from Vedrovice - probably represent contamination by modern DNA.
Sequences identified in the Kutna Hora-Denemark site (y5 thousand BP) confirm the presence of haplogroup P in the geographically partly isolated Czech basin; within Europe this haplotype has thus far been found only in aurochs. This finding (the first of its kind within the Czech Republic) is consistent with other published findings showing the domination of this auroch haplogroup line in Europe.
The combination of large individual size and 'domestic' mtDNA suggest, if inconclusively, that the early medieval fourth sample from Vysehrad could potentially represent an auroch/domestic cattle hybrid.