The Use of a Multi-Channel Capillary and a Capillary with Two Different Inner Diameters for Electrophoretic Separation of Neurotransmitters
Abstract
A fused silica capillary with seven inner channels was tested for electrophoretic experiments. An electrophoretic separation of dopamine, noradrenaline and adrenaline was performed in 100 mM acetic acid by use of contactless conductivity detection (C4D) and in 20 mM citric acid/NaOH (pH 3.2) in combination with UV detection.
Numbers of theoretical plates in multi-channel capillary are around 160000 for C4D and around 600000 for UV detection. The sensitivity in multi-channel capillary is 12times higher for C4D and 4times higher for UV detection in comparison with 25 μm single-channel capillary.
In another sets of experiments, a separation of dopamine, noradrenaline and adrenaline was completed in 18 second by use of parallel connection of 25 μm and 100 μm capillary.