An annotated and revised checklist is presented comprising 58 species (8 families) of the arachnid order Pseudoscorpiones from the Czech Republic (38 species) and Slovakia (51 species), formerly Czechoslovakia. According to the latest detailed analyses and revisions Chthonius carinthiacus Beier, 1951, Neobisium validum (L.
Koch, 1873), Roncus alpinus L. Koch, 1873 and Pselaphochernes dubius (O.
P.-Cambridge, 1892) are excluded from the checklist from Slovakia. Formerly recorded Chthonius diophthalmus Daday, 1888, C. submontanus Beier, 1963, C. kewi Gabbutt, 1966, Neobisium spelaeum (Schiodte, 1847), N. jugorum (L.
Koch, 1873), Roncus alpinus and R. transsilvanicus Beier, 1928 have not been included in the list from the Czech Republic. Some generally rare species are discussed.