Abstract
Objective: The review provides basic overview of proteomic approaches and methods and evaluates their potential towards clinical practice. Design: Review The role of laboratory biomarker assessment in patient diagnosis determination has been increasing.
Proteins unquestionably rank among very important molecules. Protein assessment of which not only enables, but can also accelerate and facilitate the diagnosis and make it more accurate.
Hence, discovery of proteins with diagnostic potential has become one of the main goals of proteomics. Currently, proteomic approaches involving separation and identification of molecules by means of advanced analytical technologies and bioinformatics are typically used for the discovery of protein and peptide biomarkers in well accessible clinical material.
However, in recent years some of the methods have been employed also for subsequent verification of the diagnostic potential of newly discovered biomarkers and are believed to be used also for routine laboratory settings in the future. The aim of the work is to provide an overview of proteomic approaches and methods and evaluate their relevance for clinical practice.