This contribution aims in characterization of structural positions of iron in human and horse spleen. 57Fe Mössbauer spectroscopy was employed as a principal method of investigation in addition to X-ray diffraction and transmission electron microscopy (TEM). At room temperature, ferritin nanoparticles exhibit superparamagnetic behavior due to their small dimensions.
Corresponding Mössbauer spectra show doublet-like patterns. Experiments performed at low temperatures unveiled presence of magnetically split components and enabled to determine the blocking temperature.
Dimensions of Fe-containing species were established from detailed analyses of TEM images.