Publication at First Faculty of Medicine |
2010
Abstract
Erythropoiesis is a multistep process regulated at the molecular level by intrinsic and extrinsic factors including microRNAs (miRNAs). We previously identified aberrant expression of miR-451 and miR-150 in polycythemia vera erythroid differentiating cells.
To address functional relevance of these miRNAs in erythroid differentiation, we employed synthetic mimics and inhibitors of miR-451 and miR-150 that we transfected into the erythroid differentiating K562 cells. We observed that enforced expression of miR-451 promoted erythroid differentiation, while inhibition of miR-150 reduced hemoglobinization of K562 cells.
Microarray data of mRNA suggested potential targets regulated by miR-451: UBE2H, ARPP-19; and by miR-150: MAS4A3, AGA, PTPRR.