Isoform-specific differences in the nitrite reductase activity of nitric oxide synthases under hypoxia

Abstract

Nitrite (NO2-) recycling to nitric oxide (NO) is catalysed by a number of enzymes and induces a protective vasodilation effect under hypoxia/ischaemia. In the present work, we tested the in vitro ability of the three NOS (nitric oxide synthase) isoforms to release NO from nitrite under anoxia using electrochemical detection, chemiluminescence and absorption spectroscopy.

Keywords

• chemiluminescence
• hypoxia
• microvascular endothelial cell
• nitric oxide synthase (NOS)
• nitrite reduction
• NO electrode
• vivo ischemia-reperfusion
• endothelial no synthase
• xanthine oxidoreductase
• crystal-structure
• reduces nitrite
• heme domain
• blood-flow
• brain
• tetrahydrobiopterin
• generation