Expression of prion protein in mouse erythroid progenitors and differentiating murine erythroleukemia cells

Abstract

The regulation of PrP(C) expression in differentiating MEL cells mimics the pattern detected on mouse erythroid precursors in vivo. Decrease of PrP(C) protein expression during MEL cell maturation is not regulated on transcriptional level.

The efficient silencing of PrP(C) levels, despite not affecting MEL cell differentiation, enables created MEL lines to be used for studies of PrP(C) cellular function.

Keywords

• prion
• hexamethylene bisacetamide
• gene expression
• blood cells
• iron uptake
• PRPC
• dexamethasone