Publication at First Faculty of Medicine |
2010
Abstract
Identification of the content of asialotransferrin in the cerebrospinal fluid is a diagnostic method for childhood-onset ataxia and central nervous system hypomyelination (CACH), also known as vanishing white matter disease (VWM), and also for other types of CNS disorders. In our work, we have determined the value of the ratio of the asialo form of transferrin to the total transferrin in the CSF using the commercially used Variant(TM) Bio-Rad system for the determination of carbohydrate-deficient transferrin (CDT) in serum.
The peak corresponding to the asialo form of transferrin was identified with electrophoresis with subsequent immunofixation and MALDI-TOF/TOF mass spectrometry.