Comparison of two different IMAC techniques used for the enrichment of phosporylated peptides

Abstract

Immobilized metal affinity chromatography (IMAC) is one of the methods used for enrichment of phosphopeptides. Two types of iminodiacetic acid (IDA)-modified sorbents loaded either with Fe(III) or Ga(III) ions were compared: magnetic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres with hydrophilic properties containing coupled IDA, and the commercial non-magnetic sorbent TopTip IMAC also containing IDA.

Keywords

• IMAC
• phosporylation