Comparison of an HPTLC method with the Reflectoquant assay for rapid determination of 5-hydroxymethylfurfural in honey
2013
Abstrakt
5-Hydroxymethylfurfural was analyzed in 17 botanical varieties of honey from 12 countries. A recently developer hight-performance thin-layer chromatographic method was limited because of increased matrix effects at higher honey sample loading.
Therefore, the method was modified to achieve higher sensitivity and eliminate matrix interference by use of rectangular application combined with a focusing step. The HPTLC results were compared with results from the new spectrophotometric Reflectoquant hydroxymethylfurfural assay.