Use of a Heme-Anchored Photolabile Probe to Explore the Active Center of Cytochrome P450 3A6

Abstract

Via its amino group, the heterobifunctional photoactivatable probe binds CYP3A6 as a heme iron ligand. After photoactivation, the probe covalently labeled CYP3A6 in tryptic peptide 167-195.

Keywords

• photoaffinity labelling
• heterobifunctional probe
• cytochrome P450
• active center