Abstract
In the present work, an interaction of four cholinesterase modulators (1–4) with calf thymus DNA was studied via spectroscopic techniques (UV–Vis, fluorescent spectroscopy and circular dichroism). From UV–Vis spectroscopic analysis, the binding constants for DNA-pyridinium oximes complexes were calculated (K = 3.5 104 to 1.4 105 M1).
All these measurements indicated that the compounds behave as effective DNA-interacting agents. Electrophoretic techniques proved that ligand 2 inhibited topoisomerase I at a concentration 5 lM.