Structure of the dimeric N-glycosylated form of fungal beta-N-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies

Abstract

The complete primary structure of the fungal beta-N-acetylhexosaminidase from Aspergillus oryzae was used to construct molecular model of the catalytic subunit of the enzyme, the enzyme dimer, and the N-glycosylated dimer.

Keywords

• substrate-assisted catalysis
• tay-sachs-disease
• molecular-dynamics
• aspergillus-oryzae
• mass-spectrometry
• aqueous-solutions
• crystal-structure
• hexosaminidase-b
• sandhoff-disease
• chitin synthesis