Abstract
The liver is a common target of toxic effect of xenobiotics. Ethical,economic,legislative,research and other reasons do not allow testing all of newly-synthesized compounds in vivo conditions.Hence new methods and approaches for hepatotoxicity testing in vitro have been developing.The most important systems for study of toxicity and metabolic activity in vitro are isolated perfused liver,liver slices,isolated liver cell in suspensions or in primary cultures including co-culture methods and special 3D techniques,various subcellular fractions and staiblised cell lines.These models can be used for cytotoxicity and genotoxicity screening,evaluation of potential hepatoprotective capacity of different compounds,study of toxic injury and characterization of hepatotoxicity mechanisms.Currently there is no an ideal in vitro liver model system for testing of hepatotoxic substances in vitro,nevertheless use of these model systems reduces economic costs and ethical and legislative problems.