Abstract
From 1997 to 2000, 134 strains of 16 yeast species from the patients with suspected or proven mycosis were evaluated in the mycology laboratory of the Department of Clinical Microbiology, Teaching Hospital in Hradec Králové. All these strains were tested by both disc test on Sabouraud agar and Fungitest.
The comparison of the results of the disk test and Fungitest showed some discrepancies which were dependent on an antifungal drug, a yeast species and the test conditions. Sabouraud agar and the criteria for interpretation of the results of the disk test seemed to be main sources of the disagreement.
A modified Mueller-Hinton agar as a recommended test medium in a proposed agar diffusion method M44-P is the way to the improvement of standardization and reproducibility of the test.