Separation and identification of carp pituitary proteins and glycoproteins

Abstract

Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and on Con A-Sepharose. The protein fractions were analyzed by SDS-PAGE and identified by N-terminal sequencing.

Keywords

• carp pituitary
• luteinizing hormone
• growth hormone
• thyroid-stimulating hornone
• oligosacharide structures