Characterization of Liposomes Used as Model System of Biological Membranes by Glassy Carbon Electrode
2013
Abstract
This contribution deals with study and characterization of liposomes. These vesicles may serve as model membranes used for study of transporting processes of heavy metal ions across the real phospholipid membranes (PLMs). 1,2-dioleoyl-sn-glycero-3-phosphocholine was used for the formation of large unilamellar vesicles (LUV).
These vesicles were prepared by hydration method. Interactions of cadmium ions with LUV in solution have been characterized using voltammetry, where glassy carbon electrode was used as the working electrode.
Size and presence of liposomes in sample was proved by dynamic light scattering and free cadmium ions in samples were determined by differential pulse voltammetry.