Abstrakt
During the development of yeast colonies internal differentiation occurs that results in the formation of various subpopulations of cells, which have different physiological and morphological characteristics. In our laboratory we recently characterised two subpopulations: L cells localized in the lower part and U cells localized in the upper part of the colony (Mol Cell 46: 436-48, 2012).
U cells specifically produce three Ato family proteins (Ato1p, Ato2p and Ato3p). The aim of this work is to identify transcription factors affecting the production of Ato proteins.
We performed high-throughput screening of Saccharomyces cerevisiae BY4742 strains carrying fluorescently labelled Ato proteins (Ato-GFP) and deletions of genes for selected transcription factors. Using colony ultra structural analysis techniques we have identified several transcription factors, which could participate in the regulation of ATO gene expression.
Obtained data will contribute to unravelling the regulatory pathways involved in the formation and development of U cell population