Cytoplasmic helicase encoded by pGKL plasmids

Abstrakt

For production of putative helicase we used T7-based system and despite of difficulty with production of AT rich sequences we successfully expressed ORF4p helicase in E. coli. Further, we partly purified ORF4p helicase by affinity chromatography

Klíčová slova

• Vopálenský Václav
• 221951710 Kluyveromyces
• linear plasmids
• two-hybrid analysis