Abstract
Mapping of DNA methylation changes especially in CpG islands has become essential for the understanding of diverse biological processes. There is an important relationship between the degree of DNA methylation and gene activity in cancer.
By discovering sites of aberrant methylation in tumors, we can identify potential new biomarkers suitable for diagnostic and prognostic testing. A number of techniques for analysis of DNA methylation have been reported.
The aim of this study was to review methodologies that can be used for monitoring of DNA methylation changes. We describe the advantages, disadvantages and potential use of these techniques.