The Cuban fauna of the genus Berosus Leach, 1817 is reviewed based on newly collected material as well as historical and type specimens. Nine species are recognized, including three recorded from Cuba for the first time: B. infuscatus LeConte, 1855, B. interstitialis Knisch, 1924 (= B. stribalus Orchymont, 1946 syn. n.) and B. metalliceps Sharp, 1882.
Only one of the nine Cuban species, B. chevrolati, remains endemic to Cuba, as two other species previously considered as endemic to Cuba are recorded from elsewhere: B. quadridens from Mexico and Central America and B. trilobus from the Dominican Republic. Notes on biology and Cuban distribution are provided for all nine species.
Berosus quadridens Chevrolat, 1863, stat. restit. is removed from synonym with B. truncatipennis and considered a valid species.