The isolated H4-H5 cytoplasmic loop of Na,K-ATPase overexpressed in it Escherichia coli retains its ability to bind ATP

Abstract

The H4-H5 loop of the alpha-subunit of mouse brain Na,K-ATPase was expressed and isolated from E.coli cells. Using fluorescence analogues of ATP! this loop was shown to retain its capability to bind ATP.

Keywords

• Tomáš Obšil
• 221951311 Na+
• K+-ATPase
• fluorescence
• ATP binding