The karyotypes and stages of meiosis of two pseudoscorpions, Semeiochernes armiger and Cordylochernes scorpioides (Pseudoscorpiones: Chernetidae), are described for the first time. The diploid chromosome number of the male is 69 in S. armiger and 47 in C. scorpioides.
As in most pseudoscorpions studied to date, autosomes exhibit predominantly metacentric morphology. Both species possess an X0 sex chromosome system.
In contrast to karyotyped European chernetids, both these neotropical species exhibit submetacentric morphology of the sex chromosomes.