Publication at Faculty of Science, Faculty of Physical Education and Sport |
2009
Abstract
Synthetic heptapeptide containing D-amino acid residues (Val-D-Leu-Pro-Phe-Phe-Val-D-Leu) was coupled to glyoxal-activated magnetic agarose particles via the free peptide amino group. The peptide-modified magnetic particles were used for the separation of pepsin A and pepsin C (porcine pepsin A, rat pepsin C and human pepsins A and C).
The elaborated method represents a rapid and simple technique not only for the separation of pepsins but also, in combination with MS, for the enzyme detection and determination.